Gram Staining & Isolation Streak Plate Method
Identification of Unknown Bacteria Laboratory Exercise


Micrococcus luteus, a Gram positive microbe isolation streak plated on TSY agar
ANAT & PHYSGENERAL BIOCELL BIOMICRO 8wkMICRO 15wkINSTRUCTORS


Lab #2: Gram Stain & Isolation Streak Plate

This is the main page for the Gram Stain & Isolation Streak Plate Laboratory Topic of an 8-week introductory college microbiology class. The links on this page provide the Virtual Microbiology Classroom documents, web pages and videos useful in preparing for this lab. 


Virtual Microbiology
Classroom






You have free access to a large collection of materials used in a college-level introductory microbiology course. The Virtual Microbiology Classroom provides a wide range of free educational resources including PowerPoint Lectures, Study Guides, Review Questions and Practice Test Questions.
Prokaryotic Cell, Mariana Ruiz
Microbiology Photo Image Library
SCIENCE PHOTOS



Photographic guides to differential stains 
now available!
1. Gram
2. Acid-fast
3. Endopsore

Application of Gram Counterstain Safranin
Instructor's Corner
SPO VIRTUAL CLASSROOMS
How to Prepare a Bacterial Smear for Gram Staining
How to Do a Gram Stain
Compound Light Microscope Parts & Operation Tutortial
MORE GRAM STAIN & ISOLATION STREAK PLATE LINKS


  • Gram Stain Bite Sized Tutorial: This is an extremely useful tutorial that shows, step-by-step, the Gram-staining procedure and the appearance of Gram+ and Gram- bacterial cells.

GRAM STAIN LABORATORY VIDEOS
GRAM STAIN LABORATORY IMAGES
The images linked here directly relate to the lab exercise and are designed to help you review and study that lab material while outside of lab. 
Microbiology Lab Videos
SCIENCE VIDEOS
NOTE: Students must review all material in categories highlighted with red titles PRIOR TO ATTENDING LAB in order to be prepared to participate. ​​​

GRAM STAIN & ISOLATION STREAK PLATE 
LABORATORY DOCUMENTS
​The Lab Exercise Instructions are the main resource required for students to understand and complete this exercise. They provide a thorough explanation of the lab topic, as well as detailed directions on how to complete the exercise. The Lab Report Assignment is the homework that students complete and turn in.

Additional Lab Documents: 

GRAM STAIN & ISOLATION STREAK PLATE LAB POWERPOINTS
The lab PowerPoints used to introduce the material in the related Lab Exercise Instructions & Report Assignment.


​​Printing: The best way for students to print out the PowerPoint Show is to download the PDF version. Select Print, and, when the Print screen comes up, go to the Print Handling options. Under Page Scaling, select "Multiple pages per sheet", Under Pages per sheet, select "2". That uses up less paper, placing two slides on each page.
The Gram Stain & Isolation Streak Plate Lab materials include:
  • Lab Exercise & Report word documents
  • Laboratory PowerPoints
  • Additional laboratory required readings
  • Videos and images  related to the lab
The purpose of isolation streak plate technique is to spread out bacteria so that individual colonies can be seen.
Page last updated: 
2/2016
Gram-negatove E. coli @ 1000xTM
Gram-positive Staphylococcus at 1000xTM
Gram negative 
E. coli @ 1000xTM
Gram positive 
Staph @ 1000xTM