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1 & 2. Acid-fast stain showing both positive (Mycobacteria) and negative (Staphylococcus) results.
Non-acid Fast Bacteria Photos (Click on image to enlarge.)
Mixed +/- Acid Fast Stain Photos (Click on image to enlarge.)
Acid Fast Bacteria Photos (Click on image to enlarge.)
1. First step of acid-fast staining procedure. Applying carbolfuchsin stain to bacterial smear and heating over water bath for 3 - 5 minutes; 2. Rinsing slide after application of crystal violet counterstain; 3. Acid-fast stained slide with + control (Mycobacterium) on left, negative contol (nonacid fast bacteria) on right and unknown in center.
Acid-fast Staining Procedure Photos (Click on image to enlarge.)
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How to Prepare a Bacterial Smear for
Acid Fast (Ziehl-Neelsen) Stain
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How to Do an Acid-fast
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Page last updated: 2/2015
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The first step of the acid-fast stain, application of carbolfuchsin, is done over a heated water bath.