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Differential Staining of Bacteria
Laboratory Exercise
Virtual Microbiology Classroom of Science Prof Online
DIFFERENTIAL STAINING LABORATORY POWERPOINT
The lab PowerPoints used in the Virtual Microbiology Classroom are designed to help introduce students to each lab project.
- Lab #2: Differential Staining of Bacteria Lab PowerPoint Show
- Lab #2: Differential Staining of Bacteria Lab Printable PDF File
Printing: The best way for students to print out the PowerPoint Show is to download the PDF version. Select Print, and, when the Print screen comes up, go to the Print Handling options. Under Page Scaling, select "Multiple pages per sheet", Under Pages per sheet, select "2". That uses up less paper, placing two slides on each page.
DIFFERENTIAL STAINING LAB DOCUMENTS
The Lab Exercise Instructions provide a thorough explanation of the lab topic, as well as detailed directions on how to complete the lab exercise. The Lab Report Template is what the students use to develop the lab report for this project.
- Differential Staining of Bacterial Cells Lab Project INSTRUCTIONS
Page last updated: 6/2015
SPO VIRTUAL CLASSROOMS
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MICROSCOPY LABORATORY IMAGES
The images linked here directly relate to the lab exercise and are designed to help students review and study that lab material while outside of lab.
HELPFUL LINKS ON DIFFERENTIAL STAINING & MICROSCOPE USE
Links to useful articles, animations and videos to help students better understand this laboratory exercise.
- How to Use A Compound Microscope Laboratory Exercise Main Page from the Virtual Microbiology Classroom.
- How to Use a Compound Microscope video by the TCCMediaCrew that is very helpful in reviewing what you learned about the microscope in lab.
- Gram Stain Bite Sized Tutorial: This is an extremely useful tutorial that shows, step-by-step, the Gram-staining procedure and the appearance of Gram+ and Gram- bacterial cells.
- Acid-fast Stain Animated Tutorial: Another good video of the staining procedure.
- Endospore Stain Procedure: Instructions, illustration and table indicating color of cells at each step.
- Bacterial Endospore Formation animation and quiz from McGraw-Hill
- Prokaryotic Cell interactive diagram from Cells Alive website.
Differential Staining of Bacterial Cells
This is the main page for the Introduction Differential Staining Topic of a 15-week introductory college microbiology class.
DIFFERENTIAL STAINING LABORATORY VIDEOS
How to Prepare a Bacterial Smear for Gram Staining
How to Prepare a Bacterial Smear for Acid-fast
(Ziehl-Neelsen) Staining
How to Prepare a Bacterial Smear for Endospore Staining
How to Do a Gram Stain
How to Do an Acid-fast
Ziehl-Neelsen Stain
How to Do an Endospore Stain
Tutorial on Compound Light Microscope Parts & Operation
The Virtual Microbiology Classroom provides a wide range of free, classroom-tested, educational resources including PowerPoint Lectures, Study Guides, Review & Practice Test Questions.
SCIENCE PHOTOS
The Microscopy Lab materials include:
- Laboratory Exercise
- Laboratory PowerPoint
- Required readings
- Videos & images of lab procedures
- Additional links that support the lab materials
NOTE: Students must review all material in categories highlighted with
red titles PRIOR TO ATTENDING LAB in order to be prepared to participate.
SCIENCE VIDEOS
Diagram on white board of bacterial controls used for Gram, Acid-fast-and Endospore differential staining of bacterial smears. Positive control on left, negative control on right, unknown bacteria in center.
DIFFERENTIAL STAINING REQUIRED LAB READINGS
These links will take students to the reading required in order to prepare for this lab exercise.
- How to Use an Inoculation Loop, Virtual Microbiology Classrooms (VMC) Lab Notes
- How to Prepare a Microscope Slide of Bacteria, VMC Lab Notes
- Differential Staining of Bacteria, VMC Lab Notes
- How to Do a Gram Stain, VMC Lab Notes
- Acid-fast (Ziehl-Neelsen) Stain, VMC Lab Notes
- Endospore Bacterial Stain, VMC Lab Notes
- Viewing Bacteria Under Oil Immersion, VMC Lab Notes
- Culture & Identification of Infectious Agents, Microbiology & Immunology On-line
- Citing References in Scientific Research Papers by Timothy Allen
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