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Science Image Library: Gram Stain
Science Image Library
Gram Stain
1. Crystal violet, the primary stain in Gram-staining; 2. Rinsing between steps of the Gram stain; 3. Application of iodine mordant during Gram stain; 4 & 5. Application of Gram counterstain, safanin.
Gram Stain Procedure (Click on image to enlarge.)
Gram Stained Slides (Click on image to enlarge.)
Gram stained slides, showing + & - bacterial controls on left and right circles of slide and unknown bacteria in center.
Gram-positive Bacteria Under Microscope
(Click on image to enlarge.)
1 & 2. Gram stain of Staphylococcus, Gram-positive @ 1000xTM; 3. Gram stain of Staphylococcus, Gram+ @ 1000xTM+; Gram+ specimen viewed at 100xTM; Gram+ specimen viewed at 400xTM.
1 & 2. Gram stain of E. coli, Gram- @ 1000xTM; 3. Gram stain of Gram-negative bacterial sample @ 1000xTM+.
SCIENCE PHOTOS
Mixed Gram + & Gram- Bacteria Under Microscope (Click on image to enlarge.)
Gram stain mixed sample od Staphylococcus (Gram +, purple cocci)
& E. coli, (Gram- pink rods) @ 1000xTM
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How to Do a Gram Stain
How to Prepare a Bacterial Smear for Gram Staining
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Gram-positive
Staphylococcus epidermidis @ 1000xTM+
Additional Gram Staining Resources
- How to Do a Gram Stain: Test for Gram+ and Gram- Bacteria Identification, "lab notes" article from Science Prof Online.
- Gram Stain Bite Sized Tutorial: This is an extremely useful tutorial that shows, step-by-step, the Gram-staining procedure and the appearance of Gram+ and Gram- bacterial cells.
Page last updated 8/2015
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SCIENCE VIDEOS
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Gram-negative Bacteria Under Microscope
(Click on image to enlarge.)
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