You have free access to a large collection of materials used in a college-level introductory microbiology course. The Virtual Microbiology Classroom provides a wide range of free educational resources including PowerPoint Lectures, Study Guides, Review Questions and Practice Test Questions.
Endospore Stain Positive Result Photos (Click on image to enlarge.)
Endospore Stain Negative Result Photos (Click on image to enlarge.)
Endospore stain of non-endospore producing bacteria. Note lack of green endospores.
Endospore Staining Procedure Photos (Click on image to enlarge.)
1, 2 & 3. Malachite green staining step of endospore stain procedure. Steam from heated water bath supplies heat to drive green stain into endospores; 4 & 5. Safranin couterstain of endospore staining procedure; 6. Endospore stained slide with + control (Bacillus) on left, negative control (E. coli) on right and unknown in center.
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