Aseptic Sterile Technique Used In Microbiology Laboratories


Aseptic Sterile Technique 
Used in Microbiology Laboratories

 

ANAT & PHYSGENERAL BIOCELL BIOMICRO 8wkMICRO 15wkINSTRUCTORS



Any medium used to grow bacteria must initially be sterile, so that the species of bacteria or clinical samples ultimately transferred to the medium remain pure and uncontaminated by microbes in the surrounding environment.
Article Summary: In a microbiology lab it's essential to avoid contamination of sterile materials and isolated cultures with the bacteria and fungal spores that are all around us.
Aseptic Technique Used to Reduce Contamination
Microincinerator used to sterilize inoculation loops
Microincinerator used to sterilize inoculation loops.
SPO VIRTUAL CLASSROOMS
Microbiology Lab Videos
SCIENCE VIDEOS
In microbiology 
aseptic technique is required, and involves being constantly mindful of each action performed in the laboratory environment. Here are some examples of how this technique is practiced.

Instructor's Corner
Page last updated: 
8/2015
Virtual Microbiology
Classroom







You have free access to a large collection of materials used in a college-level introductory microbiology course. The Virtual Microbiology Classroom provides a wide range of free educational resources including PowerPoint Lectures, Study Guides, Review Questions and Practice Test Questions.
Prokaryotic Cell, Mariana Ruiz
The SPO website is best viewed in Microsoft Explorer, Google Chrome or Apple Safari.
Sterile Media for Growing Bacteria
Bacteria grown in the lab must be provided with nutrients, moisture, the proper pH, and a surface to grow on. A growth medium (plural: media) takes care of all of these needs. 
Media is typically made from a powder that is dissolved in water and then poured into bottles and sterilized in an autoclave. 
Sterile Tryptic Soy Agar, liquid when warm, so that it can be poured into Petri dishes.
Sterile Tryptic Soy Agar, liquid when warm, so that it can be poured into Petri dishes.
TSY agar being poured into Petri dish.
Although, at this stage it is sterile (free of microbes), there is opportunity for contamination whenever the medium is interacted with, such as when it’s poured into Petri dishes or when a poured and set media is inoculated with bacterial or clinical samples. 
Continued ...
Aseptic Technique For Transferring Bacteria 
Go to PAGE 2 >
VIDEO

How to Aseptically Pour Bacterial Growth Media
Neck of TSY bottle being sterilized to prevent contamination.
Neck of TSY bottle being sterilized to prevent contamination.
CLASS NOTES 
from the free STEM 
education site 
Science Prof Online