Bacteria Growth Media & Cultures Photos

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Bacterial Growth Media & Cultures Laboratory

from Science Prof Online
ANAT & PHYSGENERAL BIOCELL BIOMICRO 8wkMICRO 15wkINSTRUCTORS


Touch plates showing bacterial growth from unwashed and washed hands

Virtual Microbiology
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You have free access to a large collection of materials used in a college-level introductory microbiology course. The Virtual Microbiology Classroom provides a wide range of free educational resources including PowerPoint Lectures, Study Guides, Review Questions and Practice Test Questions.
Prokaryotic Cell, Mariana Ruiz
1. Arm plate applied to inside of forearm to collect a bacterial sample; 2 & 3. Arm plate after incubation. Note variety of bacterial colonies.
Arm Plate Photos (Click on photo to enlarge.)
1 & 2. Touch plates: Top plate touched with dirty hands, bottom left plate touched with washed hands and bottom right plate touched with scrubbed + alcohol hands. 3. Another set of touch plates. Top plate was touched with dirty hands, bottom left plate with washed hands and bottom right plate with scrubbed + alcohol hands.​ For all photos, note that diversity of microbial growth decreases when hands are washed; 4. Close up of a touch plate showing a variety in colony morphology.
Touch Plate Photos (Click on photo to enlarge.)
1 & 2. Top and bottom view of environmental samples (kitchen counter, kitchen sink, bathroom counter, bathroom sink) plated on MacConkey's agar (left) and TSY (right); 3. Kitchen sink environmental sample plated on TSY (bottom). MacConkey's (top) shows growth of pink lactose fermenters,​ 4. Bottom view of another set of environmental samples, TSY on left and MacConkey's on right.
Environmental Samples Photos (Click on photo to enlarge.)
1. Sterile plate of Blood Agar; 2 & 3. Top and bottom view of Blood Agar plated with throat culture and showing alpha hemolysis cause by normal flora; 4. Plate of Blood Agar plated with Streptococcus pyogenes and showing beta hemolysis. 5. What a plate would look like, from bottom, of showing gamma hemolysis (no hemolysis).
Throat Swab on Blood Agar Photos (Click on photo to enlarge. See more.)
1. Sterile plate of Mannitol Salt Agar;  2. Top view of nasal culturel samples, TSY on left, MSA on right. Bacteria on MSA are normal flora; 3. Top view of two nasal cultures. Left shows growth of mannitol fermenter (agar turns yellow), right shows growth of nonmannitol fermenter (agar stays pink); 4. Bottom view of same plate; 5. Punctiform bacterial colonies of normal nasal flora Staphylococcus epidermidis.
Nasal Swab on TSY & Mannitol Salt Photos (Click on photo to enlargeSee more.)
Page last updated 5/2014
Need help remembering what the media plates looked like when they were sterile, before bacteria began to grow? 1. TSY being inoculated; 2.  Blood agar (BAP); 3. Mannitol Salt (MSA); MacConkey's (MAC); 5. Clockwise from top left: MAC, MSA BAP (bottom).
Sterile Media Photos (Click on photo to enlarge.)
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VIDEO: Mannitol Salt Agar (MSA) Specialized 
Bacterial Growth Medium
Go to > Article on Mannitol Salt Agar
VIDEO: Blood Agar (BAP) 
Bacterial Growth Medium
Go to > Article on Blood Agar
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MacConkey's Agar (MAC) Photos (Click on image to enlarge. See more)
1.Sterile MacConkey's Agar; 2. MacConkey's Agar growing Gram-negative bacteria. Left plate growing lactose-negative Salmonella, right plate growing lactose-positive E. coli; 3. "Ecoli" written on MAC using Lac+ E. coli bacteria; 4. Pink, lac+ colony growing on MAC above a tan lac- colony; 5. Smiley face of lac+, Gram- bacteria growing on MAC.
MacConkey's Agar inoculated with the following control samples (clockwise from top left): Staph aureus, Staph epi, sterile loop, Salmonella, E. coli.
Mannitol Salt Agar inoculated with the following control samples (clockwise from top left): Staph aureus, Staph epi, sterile loop, Salmonella, E. coli.
TSY Agar inoculated with the following control samples (clockwise from top left): Staph aureus, Staph epi, sterile loop, Salmonella, E. coli.
TSY, MacConkey's and Mannitol Salt Agars (left to right) inoculated with the following samples (clockwise from top left on each plate): Staph aureusStaph epi, sterile loop, SalmonellaE. coli.
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VIDEO: ​How to Interpret MacConkey's Agar (MAC) Bacterial Growth Medium
Go to > Article on MacConkey's Agar
Bacterial Controls Photos (Click on photo to enlarge.)